Integrated transcriptome and proteome analysis reveals potential mechanisms for differential abdominal fat deposition between divergently selected chicken lines

Genetic selection for meat production performance of broilers concomitantly causes excessive abdominal fat deposition, accompanied by several adverse effects, such as the reduction feed conversion efficiency and reproduction performance. Our previous studies have identified important genes regulating chicken using Northeast Agricultural University broiler lines divergently selected content (NEAUHLF) an animal model. However, molecular mechanism underlying deposition differences between lean remains largely unknown. Here, we integrated transcriptome (RNA-Seq) quantitative proteome (isobaric tags relative absolute quantitation, iTRAQ) profiling analyses on tissues from NEAUHLF lines. Differentially expressed (2167 DEGs, corrected p-value < 0.01) differentially abundant proteins (199 DAPs, 0.05) were in line compared to line. Down-regulated DEGs DAPs mainly enriched pathways related fatty acid metabolism, biosynthesis, PPAR signaling, interestingly, up-regulated both lysosome pathway. Moreover, numerous key involved long-chain uptake, situ lipogenesis (fatty cholesterol synthesis), lipid droplet accumulation discovered after analysis. Excessive critically affects health economic loss producers, but is still unclear chicken. We DEGs/DAPs potential through integration analyses. findings will facilitate a better revealing provide novel insight into discrepancy